Identification of the mutations in the prfB gene of Escherichia coli K12, which confer UGA suppressor activity.
- 1 January 1990
- journal article
- research article
- Published by Genetics Society of Japan in The Japanese Journal of Genetics
- Vol. 65 (3) , 115-119
- https://doi.org/10.1266/jjg.65.115
Abstract
By DNA sequencing and gene dissection, it has been revealed that Su+UGA #11, the mutant prfB of E. coli (Chang et al., 1990) has a double mutation compared with the wild-type LS653: one is a base substitution from T to C at the codon 63 and the other is from G to A at the codon 79. Both mutations cause amino acid substitution, Leu63 .fwdarw. Phe63 (L63F) and Asp79 .fwdarw. Gly79 (D79G), and are necessary to confer the efficient UGA suppressor activity.This publication has 9 references indexed in Scilit:
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