Simplified 13C spectral assignments using a graphical method to present 13C spectra recorded under conditions of proton off-resonance spin decoupling

Abstract
In the 13C spectrum of nicotinamide adenine dinucleotide (NAD+) the adenine and nicotinamide ring carbons are assigned by a graphical method of presenting the proton off-resonance decoupled 13C spectra as a function of proton irradiation frequency; this method is particularly applicable to closely spaced 13C signals.