Syntheses of 5′‐deoxy‐5‐[18F] fluorouridine and related compounds as probes for measuring tissue proliferation in vivo

Abstract
A rapid synthesis of 5′‐deoxy‐5‐[18F] fluorouridine (3a) from either [18F] F2 or CH3 CO2 18F is described. Fluorination of 2′,3′‐di‐O‐acetyl‐5′‐deoxyuridine (1a) with either [18F] F2 or CH3 CO2 18F in glacial acetic acid at room temperature followed by hydrolysis with sodium methoxide in methanol gives 5′‐deoxy‐5‐[18F] fluorouridine (3a) in radiochemical yields of 20‐25% in a synthesis time of 80 min from EOB. The same method also has been used to synthesize 5‐[18F] fluorouridine (3b) and 2′‐deoxy‐5‐[18F] fluorouridine (3c) in similar radiochemical yields. Acetyl hypofluorite (CH3 CO2F) is proposed as an intermediate for these reactions.

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