Role of glandular kallikrein in the activation process of human plasma inactive renin.
- 1 March 1983
- journal article
- research article
- Published by Wolters Kluwer Health in Hypertension
- Vol. 5 (2) , 191-197
- https://doi.org/10.1161/01.hyp.5.2.191
Abstract
Completely inactive renin was isolated from normal human plasma by DEAE-Sepharose column chromatography and Blue-Sepharose column chromatography. This inactive renin had a molecular weight of 54,000 daltons as determined by gel filtration on Ultrogel AcA 44. When the inactive renin was activated by trypsin, its molecular weight decreased to 48,000 daltons. The trypsin-activated renin differed from a native form of active renin in plasma with respect to molecular weight (active renin, 43,000), pI value (active renin, 5.20; trypsin-activated renin, 5.06), km value (active renin, 60 nmoles/liter; trypsin-activated renin, 89 nmoles/liter), Ki value for pepstatin A (active renin, 2.6 mumoles/liter; trypsin-activated renin 5.0 mumoles/liter) and pH profile for angiotensin formation. Glandular kallikrein (human urinary or pig pancreatic) did not activate the inactive renin. When the trypsin-activated renin was treated with glandular kallikrein, its activity was unchanged, but its molecular and kinetic properties except pI value (trypsin-activated kallikrein-treated renin, 4.82) coincided with those of a native form of active renin in plasma. These results indicate that glandular kallikrein does not directly activate inactive renin but participates in the activation process of inactive renin. The results also suggest that inactive renin in human plasma is a renin precursor.This publication has 29 references indexed in Scilit:
- Detection and isolation of inactive, large molecular weight renin in human kidney and plasma.Hypertension, 1981
- Mechanism of acid-activation of renin: role of kallikrein in renin activation.Hypertension, 1981
- Evidence for a completely inactive renin zymogen in the kidney by affinity chromatographic isolationBiochemical and Biophysical Research Communications, 1980
- A sensitive colorimetric assay for human urinary kallikreinClinica Chimica Acta; International Journal of Clinical Chemistry, 1980
- Evidence for a role of plasma kallikrein in the activation of proreninNature, 1980
- Properties of inactive renin in human plasmaClinical and Experimental Pharmacology and Physiology, 1979
- Inactive Renin — Through a Glass DarklyNew England Journal of Medicine, 1979
- KALLIKREIN AND PLASMIN AS ACTIVATORS OF INACTIVE RENINThe Lancet, 1978
- Human urinary kallikrein converts inactive to active renin and is a possible physiological activator of reninNature, 1978
- Separation of human renin substrate from renin and a major contaminating albumin using a concanavalin A-Sepharose columnClinica Chimica Acta; International Journal of Clinical Chemistry, 1977