Rapid Micropropagation of Paulownia tomentosa

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Abstract
Nodes from mature tissue of Paulownia tomentosa Steud. were cultured on a modified Murashige-Skoog (MS) medium containing BAP and NAA. Axillary buds elongated most rapidly from nodes of greenhouse-grown trees on a medium containing 1.0 mg BAP/liter and 0.1 mg NAA/liter. After 7 days on this medium, the elongated shoots were transferred to media containing IBA (0.5–1.0 mg/liter) or the shoots were quick-dipped (15 sec) in the K-salt of IBA (KIBA, 500–1000 ppm) and rooted under mist in the greenhouse. The shoots rooted in 7–10 days and were easily transplanted to 6 packs (6-celled plastic flat) for continued development. This propagation scheme provides a method for the rapid multiplication of plants from mature tissue Paulownia in a very short period of time (14–17 days). Chemical names used: 1-naphthaleneacetic acid (NAA); benzylaminopurine (BAP); 1H-indole-3-butanoic acid (IBA).

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