Plant regeneration from protoplasts isolated from long-term cell cultures of wheat (Triticum aestivum L.)
- 1 March 1991
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 9 (11) , 611-614
- https://doi.org/10.1007/bf00231799
Abstract
A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yield of protoplasts was released from suspension cells (2 to 3×107 protoplasts per ml packed cell volume) when treated with enzyme mixtures. The isolated protoplasts divided at a relatively high frequency (20% to 50%) in both liquid and agarose-solidified KM8p medium. Up to 0.21% of the dividing protoplasts continued to divide and form micro-calli. Sixty-eight plants were regenerated from micro-calli, and among the 30 plants which were transplanted to the greenhouse, 3 have survived.Keywords
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