Spectrofluorometric Determination of Atropine-Hyoscyamine in Tablets and Powders

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Abstract
A method is presented for determining atropine-hyoscyamine alkaloids in tablets and powders by spcetrofluorometry. The sample is first shaken with 0.1N H2S04 solution; then an aliquot containing about 0.1 mg alkaloid is transferred to a separatory funnel, made basic with NaOH solution, and extracted with CHC13. The extract is added to a Celite-0.2N H2S04 column and the eluate is discarded. The alkaloids are then eluted from the column with ammoniacal CHCI3 and the eluate is evaporated to dryness. The residue is dissolved in CHC13, eosin Y solution is added, and the solution is mixed. Aliquots of a reference solution of atropine are similarly treated and a blank is prepared from CHCI3 and eosin Y solution. All solutions are scanned with a recording spectrofluorometer in the emission mode from 440 to 700 nm and the excitation set at 475 nm. The emission peaks near 550 nm are used for quantitative estimation of the alkaloids. The method cannot generally be used in the presence of other organic bases but is applicable in the presence of barbiturates.

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