Biosynthesis of heteroscyphic acid A in cell cultures of Heteroscyphus planus: nonequivalent labelling of C-5 units in diterpene biosynthesis

Abstract
Preferential labelling in the farnesyl diphosphate (FPP)-derived portion and a 1,2-migration of the methyl (C19) originating at C-6 of mevalonate in the biosynthesis of heteroscyphic acid A {(12Z)-5,10-trans-cleroda-3,12,14-trien-20-oic acid} incorporating [2-13C]- and [4,5-13C2]-mevalonates are evident from the 13C-enrichment and the complete 13C-13C coupling pattern.
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