Electrochemically Addressable Functionalization and Parallel Readout of a DNA Biosensor Array
- 28 February 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 79 (7) , 2695-2702
- https://doi.org/10.1021/ac061678l
Abstract
We introduce an electrochemical method for the directed in situ assembly of various specific oligonucleotide capture probes onto different sensing elements of a multielectrode array in the aqueous environment of a flow cell. Surface plasmon microscopy is utilized for the on-line recording of the various functionalization steps. Hybridization reactions between targets from solution to the different surface-bound complementary probes are monitored by surface plasmon field-enhanced fluorescence microscopy using targets that are either labeled with organic dyes or with semiconducting quantum dots for color-multiplexing. This study provides a new approach for the fabrication of (small) DNA arrays and the recording and quantitative evaluation of parallel hybridization reactions.Keywords
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