The analysis of mock DNA sequencing reactions using matrix‐assisted laser desorption/ionization mass spectrometry
- 1 October 1993
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 7 (10) , 895-897
- https://doi.org/10.1002/rcm.1290071008
Abstract
A mock sequencing experiment is described in which matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry is used to analyze mixtures of synthetic oligodeoxynucleotides chosen to match the first 24 DNA fragments generated in typical sequencing reactions using a standard M13mp19 template and primer. The experiments permitted the evaluation of important issues relating to resolution, sensitivity, multiple charging, and adduct formation. Mass resolutions (full width at half maximum) were between 100 and 200, and it was relatively straightforward to assign bases in the sequencing simulation using subpicomolar quantities of material. These results demonstrate the potential of MALDI mass spectrometry in DNA sequence analysis.Keywords
This publication has 7 references indexed in Scilit:
- Matrix‐assisted laser desorption time‐of‐flight mass spectrometry of oligonucleotides using 3‐hydroxypicolinic acid as an ultraviolet‐sensitive matrixRapid Communications in Mass Spectrometry, 1993
- Laser mass spectrometry of polydeoxyribothymidylic acid mixturesRapid Communications in Mass Spectrometry, 1993
- Solid-phase method for the purification of DNA sequencing reactionsAnalytical Chemistry, 1992
- Matrix‐assisted laser desorption/ionization mass spectrometry of synthetic oligodeoxyribonucleotidesRapid Communications in Mass Spectrometry, 1992
- Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of BiopolymersAnalytical Chemistry, 1991
- Rapid, sensitive analysis of protein mixtures by mass spectrometry.Proceedings of the National Academy of Sciences, 1990
- Ultraviolet-laser desorption/ionization mass spectrometry of femtomolar amounts of large proteinsJournal of Mass Spectrometry, 1989