Presence of Protein I, a Phosphoprotein Associated with Synaptic Vesicles, in Cerebellar Granule Cells

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Abstract
The cerebellar levels of Protein I, a synapse‐specific neuronal phosphoprotein, have been investigated in the cerebellar mouse mutants staggerer (sg), weaver (wv), nervous (nr), and Purkinje cell degeneration (pcd). The Protein I concentration was reduced by about 66% in sg and wv mutants, representing a 90% loss of Protein I per cerebellum. A heterozygote effect was observed in the wv mutant. These results indicate that a great majority of Protein I in the normal cerebellum may be present in the granule cells. in nr mutants the cerebellar Protein I concentration was reduced by only 12% in 62‐day‐old mice, suggesting that Purkinje cells contribute little to cerebellar Protein I. However, a greater reduction was observed in pcd mutants, which may reflect on the nature of the pcd mutation.