Intestinal Excretion of Cholesterol and Total Lipids by the Nephrotic Rat

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Abstract
Adult male Long-Evans strain rats (8) were made nephrotic by injecting each intravenously with 1 ml of anti-rat-kidney serum. Five days later each was bled, along with 5 control animals, and plasma total lipids and total cholesterol determined. All rats were then placed in individual cages for 24 hours without food, the 24 hour fecal excretion collected, the animals killed and the entire intestinal tract distal to the pylorus stripped of its contents, which were added to the respective stool collection. The combined stool sample was analyzed for lipids and cholesterol. Nephrotic rats showed plasma cholesterol averaging 483 mg%, the controls 69 mg%. The nephrotic rats showed average plasma lipid of 1240 mg%, the controls 213 mg%. Stool content of cholesterol in the nephrotic rats averaged 8.6 mg/24 hours; the controls 6.1 mg/24 hours. Stool content of lipid in the nephrotic rats averaged 192 mg/24 hours, that of the controls 172 mg/24 hours. Experimental nephrotic hypercholesteremia and hyperlipemia apparently cannot be ascribed to a diminished intestinal excretion of these substances.