Virion-associated RNA polymerase required for bacteriophage N4 development.
Open Access
- 1 February 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (2) , 520-523
- https://doi.org/10.1073/pnas.74.2.520
Abstract
Escherichia coli phage N4 transcription is resistant to rifampicin, an inhibitor of the host RNA polymerase, even when the drug is added prior to infection. A rifampicin-resistant RNA polymerase was detected in disrupted N4 virions. This enzyme shows a requirement for the 4 ribonucleoside 5''-triphosphates and exogenous denatured DNA. With denatured N4 DNA, the preferred template, transcription is asymmetric. The virion RNA polymerase apparently is necessary for phage development, because a conditional lethal N4 mutant shows temperature-sensitive RNA synthesis in vivo and a temperature-sensitive RNA polymerase in disrupted virions.This publication has 21 references indexed in Scilit:
- Rifampicin-resistant RNA polymerase and NAD transferase activities in coliphage N4 virionsNature, 1976
- New RNA polymerase from Bacillus subtilis infected with phage PBS2Nature, 1974
- Novel transcribing activities in N4-infected Escherichia coliVirology, 1974
- Purification of Ribonucleic Acid Polymerase from SP82-infected Bacillus subtilisJournal of Biological Chemistry, 1974
- RNA Polymerase from Bacillus amyloliquefaciens Infected with ϕ29 BacteriophageProceedings of the National Academy of Sciences, 1973
- Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme.1973
- Selective shutoff of catabolite-sensitive host syntheses by bacteriophage N4Virology, 1972
- Resistance of bacteriophage PBS2 infection to rifampicin, an inhibitor of Bacillus subtilis RNA synthesisBiochemical and Biophysical Research Communications, 1972
- Different Template Specificities of Phage T3 and T7 RNA PolymerasesNature New Biology, 1971
- Role of host RNA polymerase for λ phage developmentBiochemical and Biophysical Research Communications, 1969