Linking integrin to IP3 signaling is important for ovulation in Caenorhabditis elegans
- 22 December 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (2) , 549-553
- https://doi.org/10.1016/j.febslet.2004.12.026
Abstract
Signals from germ and myoepithelial sheath cells initiate ovulation in Caenorhabditis elegans. The coordinated dilation and contraction of spermatheca lead to subsequent fertilization of oocyte. Either the dominant negative mutant pat‐3 β integrin or disruption of talin expression block ovulation [Cram, E.J., Clark, S.G. and Schwarzbauer, J.E. (2003) Talin loss‐of‐function uncovers roles in cell contractility and migration in C. elegans. J. Cell. Sci. 116, 3871–3878; Lee, M., Cram, E.J., Shen, B. and Schwarzbauer, J.E. (2001) Role of β pat‐3 integrins in development and function of Caenorhabditis elegans muscles and gonads. J. Biol. Chem. 276, 36404–36410], suggesting that the interaction between the cell and the extracellular matrix (ECM) is also important for ovulation. Here, we report that integrin plays an essential role in fertility via IP3 signaling. Sterility caused by RNAi of pat‐3 and ECM molecules was suppressed by increased IP3 signaling. Our data suggest that the cell–ECM interaction controls ovulation via IP3 signaling.Keywords
This publication has 24 references indexed in Scilit:
- Inositol 1,4,5-Trisphosphate Signaling Regulates Rhythmic Contractile Activity of Myoepithelial Sheath Cells inCaenorhabditis elegansMolecular Biology of the Cell, 2004
- Tropomyosin and Troponin Are Required for Ovarian Contraction in theCaenorhabditis elegansReproductive SystemMolecular Biology of the Cell, 2004
- Genome-Wide RNAi of C. elegans Using the Hypersensitive rrf-3 Strain Reveals Novel Gene FunctionsPLoS Biology, 2003
- Talin loss-of-function uncovers roles in cell contractility and migration inC. elegansJournal of Cell Science, 2003
- Systematic functional analysis of the Caenorhabditis elegans genome using RNAiNature, 2003
- IntegrinsCell, 2002
- Phospholipase Cγ Binds α1β1Integrin and Modulates α1β1 Integrin-specific AdhesionJournal of Biological Chemistry, 2002
- On the Role of RNA Amplification in dsRNA-Triggered Gene SilencingCell, 2001
- Roles for βpat-3 Integrins in Development and Function ofCaenorhabditis elegans Muscles and GonadsJournal of Biological Chemistry, 2001
- Inositol 1,4,5-trisphosphate receptors are strongly expressed in the nervous system, pharynx, intestine, gonad and excretory cell of Caenorhabditis elegans and are encoded by a single gene (itr-1)Journal of Molecular Biology, 1999