Subcellular distribution of α, and β subunit proteins of Ca2+ /calmodulin‐dependent protein kinase II expressed in Chinese hamster ovary cells
- 18 June 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 266 (1-2) , 55-58
- https://doi.org/10.1016/0014-5793(90)81505-i
Abstract
When two cDNAs respectively encoding the entire coding regions of α and β subunits of Ca2+ /calmodulin-dependent protein kinase II (CaM kinase II) were introduced into Chinese hamster ovary cells, the expressed α and β subunits were differently associated with subcellular structure. Although α subunit was loosely associated with subcellular structure, about 80% of CaM kinase II acitivity of α subunit was found in soluble fraction. More than 50% of the β subunit bound to the membrane, and the remainder was soluble but was loosely associated with subcellular structure. The relative rate of phosphorylation for substrate proteins of the β subunit bound to membrane was significantly different from that of the soluble form.Keywords
This publication has 17 references indexed in Scilit:
- A Retinal Calmodulin‐Dependent Kinase: Calcium/ Calmodulin‐Stimulated and ‐Inhibited StatesJournal of Neurochemistry, 1988
- Two Developmentally Regulated Isoenzymes of Calmodulin‐Stimulated Protein Kinase II in Rat ForebrainJournal of Neurochemistry, 1988
- Developmental Changes in Calmodulin‐Kinase II Activity at Brain Synaptic Junctions: Alterations in Holoenzyme CompositionJournal of Neurochemistry, 1987
- Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.Proceedings of the National Academy of Sciences, 1987
- Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning.Proceedings of the National Academy of Sciences, 1987
- Calcium · calmodulin-dependent protein kinase II and calcium · phospholipid-dependent protein kinase activities in rat tissues assayed with a synthetic peptideArchives of Biochemistry and Biophysics, 1987
- PROTEIN KINASES IN THE BRAINAnnual Review of Biochemistry, 1985
- Evidence that the major postsynaptic density protein is a component of a Ca2+/calmodulin-dependent protein kinase.Proceedings of the National Academy of Sciences, 1984
- Purification and Cahracyterization of te Brain Calmodulin‐Dependent Protein Kinase (Kinase II), Which Is involved in the Activtion of Tryptophan 5‐MonooxygnaseEuropean Journal of Biochemistry, 1983
- Evidence for three distinct forms of calmodulin‐dependent protein kinases from rat brainFEBS Letters, 1980