Revised classification of the DLA loci by serological studies
- 1 May 1986
- journal article
- research article
- Published by Wiley in Tissue Antigens
- Vol. 27 (5) , 262-268
- https://doi.org/10.1111/j.1399-0039.1986.tb01531.x
Abstract
Peripheral blood mononuclear cells from DLA typed dogs were treated with rabbit-anti-dog-.beta.2-microglobulin and subsequently with goat-anti-rabbit-immunoglobulin in order to aggregate the DLA class I molecules on the cell membrane (lysostrip). Utilizing a panel of 70 defined DLA-A and DLA-B antisera, lymphocytes treated in this way showed resistance to complement dependent lysis with monospecific DLA-A sera only, whereas reactivity of DLA-B antisera was not blocked; on the contrary, complete lympholysis with each DLA-B antiserum was recognized. Thus, the DLA-B antigens, evidently not associated with .beta.2-microglobulin, are designated as candidates for class II gene products. The different reactions of DLA-C antisera after lysostrip did not allow a precise assignment of this antigen series as yet.This publication has 13 references indexed in Scilit:
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