Immunochemical Characteristics of Isoperoxidases from Two Environmentally-induced Flax Genotrophs (Linum usitatissimumL.)

Publisher Website
Google Scholar
Abstract
The homology between the acidic isoperoxidases from two environmentally-induced flax genotrophs, L and S, was examined with antisera raised against purified isozymes from S stem tissue. Peroxidases S1, S2 and S4 were found to be immunologically indistinguishable from their counterparts L1, L2 and L4 based on results from immunodiffusion, immunoinhibition and immunoprecipitation experiments. Corresponding isozymes from S and L, despite displaying differences in apparent molecular weight, were shown to have identical pl values. These results support our view that post-translational modification of the carbohydrate moiety of the isoperoxidases from L and S is responsible for their differences on polyacrylamide gel electrophoresis. The affinity of the antisera toward three horseradish peroxidases was also studied and the presence of three antigenically distinct groups of peroxidases in plants is suggested.