Inhibition of Leukocyte Migration by Tumor-Associated Antigens in Soluble Extracts of Human Breast Carcinoma 2

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Abstract
A leukocyte migration-inhibition (LMI) assay was developed to detect cell-mediated immune reactions to tumor-associated antigens (TAA) of human breast carcinoma. The test initially used standard streptokinase/ streptodornase and purified protein derivative antigens and leukocytes from individuals who were either skin-test positive or negative to these antigens. Generally, the results of the LMI assay correlated well with in vivo positive and negative skin reactions and demonstrated that the test could detect de!ayed hypersensitivity. To determine whether the assay could detect TAA of human breast carcinomas, tests were done on leukocytes from patients with breast carcinoma, benign breast disease, and cancer of other sites. Normal controls were also tested. Antigens were obtained by 3M KCI extraction of breast carcinoma, benign breast tissues, and normal breast tissues, Most tests were allogeneic; 2 were autologous. Migration of leukocytes from 20/26 breast carcinoma patients was markedly inhibited by KCI extracts of breast carcinoma. No leukocytes tested by the LMI assay against KCI extracts of normal or benign breast tissue were significantly inhibited. Infrequently, leukocytes of patients with carcinoma of other sites were inhibited by soluble KCI extracts of breast carcinoma. Allogeneic reactions suggested that different breast carcinomas had common antigens.

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