STUDIES ON THE PHYSIOLOGY OF RICKETTSIAE IV

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Abstract
Mattheis, MarthaS. (University of Kansas, Lawrence), M.Silverman, and D. Paretsky. Studies on the physiology of rickettsiae. IV. Folic acids ofCoxiella burnetii. J. Bacteriol.85:37–41. 1963.—Yolk, yolk sac, and embryo tissues of uninfected eggs, and those infected withCoxiella burnetii, were analyzed for folic acid derivatives by employing diethylaminoethyl (DEAE)-cellulose column chromatography. Infected tissues contained quantitatively less folate, but the elution profiles of both infected and uninfected tissues were identical. PurifiedC. burnetiicontained some types of folate apparently unique to these rickettsiae, and not found in infected tissue. The major folate fraction ofC. burnetiiwas partially characterized by (i) elution position from DEAE columns; (ii) treatment with conjugase; (iii) growth response byLactobacillus casei, Streptococcus faecalisR, andPediococcus cerevisiae; and (iv) response to oxidation, reduction, and formylation.