Common Antigens of Streptococcal and Non-streptococcal Oral Bacteria: Isolation and Biochemical Characterization of the Extracellular Protein Antigen
- 1 September 1982
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 128 (9) , 2113-2120
- https://doi.org/10.1099/00221287-128-9-2113
Abstract
An extracellular soluble common protein (ECP) was purified from extracellular soluble fractions of exponential phase cultures of Streptococcus sanguis OMZ9, of a representative strain of each of Bratthall''s 7 serological groups of S. mutans and of 1 strain each of Lactobacillus salivarius and Actinomyces viscosus. The ECP antigens from the different strains were prepared from SDS[sodium dodecyl sulfate]-dissociated immunoprecipitates by affinity chromatography on an anti-rabbit Ig column. The identity of such purified ECP antigens was demonstrated by their behavior in immunodiffusion analysis, in SDS-PAGE [polyacrylamide gel electrophoresis], in which an identical MW (60,000) was found and by virtue of their similar amino acid and sugar compositions. This ECP consisted of 90% protein and 10% sugar.This publication has 2 references indexed in Scilit:
- Common antigens of streptococcal and non-streptococcal oral bacteria: immunochemical studies of extracellular and cell-wall-associated antigens from Streptococcus sanguis, Streptococcus mutans, Lactobacillus salivarius, and Actinomyces viscosusInfection and Immunity, 1981
- Characterisation of antigens extracted from cells and culture fluids of Streptococcus mutans serotype cArchives of Oral Biology, 1978