Immunopharmacological actions of Neurotropin (1)

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Abstract
The immunostimulating actions of NSP [neurotropin] were studied. NSP alone did not induce a proliferation of spleen cells from mice or human peripheral blood lymphocytes (PBL). NSP clearly enhanced concanavalin A(Con A)-induced proliferation of both spleen cells and human PBL, but slightly inhibited lipopolysaccharide(LPS)-induced proliferation of spleen cells. NSP reversed the decreases of Con A-induced proliferation of spleen cells pretreated with mitomycin C (MMC) in a dose of 5 .mu.g/ml or irradiated at 100-500 R, while NSP did not reverse the decreases of LPS-induced proliferation of spleen cells pretreated with MMC, in a dose of 5-50 .mu.g/ml, or irradiated at 100-1000 R. NSP enhanced in a dose-dependent fashion 1-way mixed lymphocytes culture (MLC) reaction using MMC-treated spleen cells from BDF1 mice as stimulator cells and those from C57BL/6 mice as responder cells, respectively. NSP reversed the considerable decrease of MLC-mediated proliferation of responder cells pretreated with MMC, in a dose of 5-10 .mu.g/ml, or irradiated at 100-250 R. NSP activates T cell function participating in cell-mediated immunity.