Distribution of Heavy‐Chain Variable‐Region (VH) Subgroups on Human Lymphocytes

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Abstract
Lymphocytes from 20 normal blood donors were stained with fluorescein isothiocyanate (FITC)-conjugated anti-F(ab'')2, anti-VHI, anti-VHII and anti-VHIII subgroup antisera. The means of the percentages of staining were 2.9%, 5.0% and 5.5% for the VHI, VHII and VHIII subgroups, respectively. The sum of the percentages of the lymphocytes stained with each of the VH subgroup-specific antisera corresponded well with the percentages of lymphocytes stained with an anti-F(ab'')2 antiserum. It was shown by double immunofluorescence staining and in various depletion experiments and tests with thymocytes and lymphocytes from patients with hypogammaglobulinemia that the cells staining with anti-VH antisera corresponded to the membrane Ig-positive cells, i.e., the B[bone marrow-derived]-lymphocyte population.

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