Criticism of a Technic Claimed to Demonstrate Mitotic Spindles in Bacteria
- 1 January 1953
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 28 (1) , 45-48
- https://doi.org/10.3109/10520295309112635
Abstract
A technic of staining bacteria with acid-hydrolysed thionin and dehydrating in freezing alcohol, claimed by DeLamater (1951) to be specific for nuclear structures, to preserve the bacterial cell unaltered and to reveal mitotic spindles in Bacillus, is shown to cause considerable distortion, and to produce stained artifacts, de- rived from the transverse septa of this organism. It is these artifacts which have been confused with mitotic figures by DeLamater.Keywords
This publication has 2 references indexed in Scilit:
- SOME MORPHOLOGICAL CHANGES CAUSED BY VARIOUS TREATMENTS OF BACTERIA, AS STUDIED BY ELECTRON MICROSCOPYActa Pathologica Microbiologica Scandinavica, 1951
- A Staining and Dehydrating Procedure for the handling of MicroörganismsStain Technology, 1951