New Pathway for Long-Chain n -Alkane Synthesis via 1-Alcohol in Vibrio furnissii M1
- 15 February 2005
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 187 (4) , 1426-1429
- https://doi.org/10.1128/jb.187.4.1426-1429.2005
Abstract
Alkane biosynthesis in the bacterium Vibrio furnissii M1 involves the synthesis of long-chain alkanes via 1-alcohol. Evidence for this novel pathway are the following. (i) Both even- and odd-carbon-number n -alkanes were produced from glucose, while only even-carbon-number fatty acids were produced in V. furnissii M1. This result cannot be explained by the decarbonylation pathway. (ii) Pentadecane and hexadecane were produced from 1-hexadecanoic acid by membrane fractions of V. furnissii M1, and radioisotope precursor-tracer experiments, in which 1-[1- 14 C]hexadecanoic acid was fed, identified the corresponding alcohol, aldehyde, and alkane derivatives. Since all metabolites maintained the radioisotope label at 1-C, they were produced by a pathway in which the carbon structure was retained, i.e., a reduction pathway. (iii) n -Hexadecane was produced when 1-hexadecanol was fed to membrane preparations.Keywords
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