Regulation of the H+/e− stoichiometry of cytochrome c oxidase from bovine heart by intramitochondrial ATP/ADP ratios
- 11 March 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 382 (1-2) , 121-124
- https://doi.org/10.1016/0014-5793(96)00096-8
Abstract
This paper describes the effect of intramitochondrial ATP/ADP ratios on the H+/e− stoichiometry of reconstituted cytochrome c oxidase (COX) from bovine heart. At 100% intraliposomal ATP the H+/e− stoichiometry of the reconstituted enzyme is decreased to half of the value measured below 98% intraliposomal ATP (above 2% ADP), while it remains constant up to 100% ADP. The decrease is obtained with different COX preparations, independent of the absolute value of the H+/e− stoichiometry. Decrease of H+/e− stoichiometry is prevented by preincubation of the enzyme with a tissue‐specific monoclonal antibody to subunit VIa‐H (heart‐type). Tissue‐specific regulation of the efficiency of energy transduction in COX of muscle mitochondria could have a physilogical function in maintaining the body temperature at rest or sleep, i.e. at low ATP expenditure.Keywords
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