Arachidonic acid induces phosphorylation of an 18 kDa protein in electrically permeabilised rat islets of Langerhans
- 13 January 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 296 (1) , 69-72
- https://doi.org/10.1016/0014-5793(92)80405-6
Abstract
Arachidonic acid (AA) was shown to induce concentration-dependent, calcium-independent, in situ phosphorylation of a protein of approximate molecular weight 18 kDa in electrically permeabilised rat islets of Langerhans. This protein did not appear to be a substrate for protein kinase C (PKC) since stimulation of PKC by 4β phorbol myristate acetate (4β PMA) did not result in 32P incorporation into an 18 kDa protein, and since AA-induced phosphorylation was observed in islets in which PKC had been down-regulated by prolonged exposure of islets to 4β PMA. These results suggest that AA stimulates protein phosphorylation by a mechanism other than PKC activation.Keywords
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