Zonal centrifuge analysis of a transformation in Ustilago controlled by zinc.

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Abstract
Zonal centrifugation was employed to separate intracellular particles in the sporidia of the smut fungus, Ustilago sphaerogena. Sporidia were obtained in 2 states, corresponding to media containing high (2x10-5 [image]) and low (4x10-7 [image]) Zn. The high-Zn cells produced much more cytochrome c than low- Zn cells. High-Zn cells were distinguished by large amounts of heavy particles, possibly mitochondria, which appeared as a separate peak in both rate-zonal and isopycnic separations. Ribosome peaks were present and indistinguishable in breis from the 2 kinds of cells. Problems encountered with the zonal centrifugation of these yeast-like organisms are discussed.