Function of chloroplast DNA. I. Hybridization studies involving nuclear and chloroplast DNA with RNA from cytoplasmic (80S) and chloroplast (70S) ribosomes.
Open Access
- 1 February 1968
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 59 (2) , 569-576
- https://doi.org/10.1073/pnas.59.2.569
Abstract
Hybridization experiments were performed with chloroplast and nuclear DNA''s and H3-RNA''s isolated from highly purified 70S chloroplast and 80S cytoplasmic ribosomes from tobacco leaves. Chloroplast DNA hybridized with chloroplast ribosomal RNA (0.5%) but not with cytoplasmic ribosomal RNA. Nuclear DNA hybridized with cytoplasmic riboscmal RNA (0.25%) and also with chloroplast ribosomal RNA (0.1%). The coding information contained in nuclei for chloroplast ribosomal RNA is about 3 times greater than that contained in chloroplast DNA on a per cell basis. The chloroplast ribosomal RNA cistrons contained in nuclear DNA occupy sites separated from the many repetitive cistrons for cyto-plasmic ribosomal RNA.This publication has 19 references indexed in Scilit:
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