Determination of sulfobromophthalein storage and exertory rate in small animals

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Abstract
The hepatic removal of sulfobromophthalein (BSP) from plasma involves both storage and excretion. A method has been devised for the simultaneous determination of relative storage capacity (S) and transport maximum (Tm) in small laboratory animals. Comparable values were obtained in dogs by the proposed method when compared to the method presently used in dogs and humans (Wheeler''s method), thus substantiating the use of the new proposed method in small animals. Similar results were obtained for Tm and S when two different rates of BSP infusion were used in rabbits and rats. This is in agreement with what one would expect if the proposed method were validly determining Tin and S. The following Tm values (mg/min per kg) were obtained with this method in anesthetized animals: dog, 0.17; rabbit, 1.05; and rat, 0.97. The following S values (mg/mg/100 ml per kg) were simultaneously obtained: dog, 2.4; rabbit, 0.55; and rat, 0.47.