Endocarditis Caused by Culture-Negative Organisms Visible by Brown and Brenn Staining: Utility of PCR and DNA Sequencing for Diagnosis
- 1 May 2001
- journal article
- case report
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (5) , 2025-7
- https://doi.org/10.1128/jcm.39.5.2025-2027.2001
Abstract
Two cases of culture-negative endocarditis with cocci seen in valve vegetations are presented. The organisms were identified by molecular analysis using broad-range PCR primers complementary to the 16S rRNA gene, sequencing, and database search using BLAST software. The results and utility of this method are discussed.Keywords
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