Plaque Production with Infectious Bovine Rhinotracheitis (IBR) Virus.

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Abstract
Summary and conclusions Plaques were produced by IBR virus on bovine embryo kidney cells by both Dulbecco's agar-overlay and Postlethwaite's liquid overlay methods. The lower plaque titers obtained when the latter method was used were perhaps due to errors in scoring plaques which were not clearly distinct. The formation of plaques by IBR virus was blocked by IBR hyperimmune serum in both the standard tube neutralization test and by incorporation of antiserum into agar-overlay. Plaquing of IBR virus on bovine embryo kidney cells provided a more quantitative means by which to study the host-range of this virus and a more precise system for the biological assay of infective IBR nucleic acids which may possibly be obtained from the virus.

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