Experimentelle Studien über den Nucleinstoffwechsel. XXXV. Die Pyrimidinnucleotide aus Thymusnucleinsäure.

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Abstract
XXXV. Thymus nucleic acid was enzymically hydrolized to mononucleotides by previously described methods. Of these nucleotides, desoxyribose-guanylic acid and desoxyribose-adenylic acid had been previously isolated. From the mother liquor from which the brucine salt of the latter was obtained, by fractional crystallization of brucine and barium salts, desoxyribose-cytidylic acid and thymosine phosphoric acid were now isolated. The identification of both of these compounds was verified by enzymic dephosphorylation and isolation of the nucleosides. All 4 of the nucleotides of thymus nucleic acid have therefore now been isolated and crystallized.[long dash]XXXVI. Takahashi''s results, which supported a closed ring ester structure for nucleic acids, "could not be reproduced. The existence of a specific thymonucleinase, distinct from phosphatase, and splitting nucleic acid only to the mononucleotide stage, was the chief basis for the rejection of the ester structure, and support of the anhydride structure.[long dash]XXXVII. Nucleosidase. which split nucleosides into carbohydrate and purine (or pyri-midine), was found in most tissues. Its preparation and purification from spleen is described. Purified nucleosidase was most active at pH 6.5, and acted only in the presence of arsenate or phosphate. A method is described for the estimation of the enzyme.

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