Description ofMycobacterium conceptionensesp. nov., aMycobacterium fortuitumGroup Organism Isolated from a Posttraumatic Osteitis Inflammation

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Abstract
A nonpigmented rapidly growing mycobacterium was isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. The three isolates grew in 3 days at 24 to 37°C. 16S rRNA sequence analyses over 1,483 bp showed that they were identical and shared 99.7% (4-bp difference) sequence similarity with that ofMycobacterium porcinum, the most closely related species. PartialrpoB(723 bp) sequence analyses showed that the isolates shared 97.0% sequence similarity with that ofM. porcinum. Further polyphasic approaches, including biochemical tests, antimicrobial susceptibility analyses, andhsp65,sodA, andrecAgene sequence analysis, as well as % G+C determination and cell wall fatty acid composition analysis supported the evidence that these isolates were representative of a new species. Phylogenetic analyses showed the close relationship withM. porcinumin theMycobacterium fortuitumgroup. The isolates were susceptible to most antibiotics and exhibited evidence for penicillinase activity, in contrast toM. porcinum. We propose the nameMycobacterium conceptionensesp. nov. for this new species associated with posttraumatic osteitis. The type strain is D16T(equivalent to CIP 108544Tand CCUG 50187T).

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