Solubilisierung und Anreicherung einer 3α-Hydroxysteroid-Dehydrogenase aus Rattenleber-Mikrosomen

Abstract
The microsomal 3-hydroxysteroid dehydrogenases were solubilized with lubrol, a non-ionic detergent. A 3.alpha.-hydroxysteroid dehydrogenase (EC 1.1.1.50) was purified about 100-fold by double affinity chromatography on 5.alpha.-dihydrotestosterone-Sepharose. This enzyme can use both NADH and NADPH as coenzymes.