Simian virus 40 large tumor antigen: a "RNA binding protein"?

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Abstract
SV40 large tumor antigen [TAg] was isolated by immunoaffinity chromatography from monkey or mouse cell cultures undergoing lytic or transforming infection. RNase-treated gel-purified TA, on hydrolysis with alkali, gave about equimolar amounts of AMP, GMP, CMP and UMP. RNA fragments of .apprxeq. 45 nucleotides could be isolated from TA purified by the same procedure. Mapping of the T1 oligonucleotides showed a high complexity, as indicated by the presence of unique sequences of 15-30 nucleotides and of poly(A). These RNA fragments apparently are derived from celluar pre-mRNA or mRNA. SV40 TA appears to be a RNA-binding protein and might possibly be involved in regulation of synthesis, maturation or translation of cellular mRNA.