Calcium-Regulated Plasma Membrane Rigidification During Corpus Luteum Regression in the Rat
- 1 February 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 32 (1) , 77-82
- https://doi.org/10.1095/biolreprod32.1.77
Abstract
Plasma membrane fractions were prepared from ovaries of superovulated rats and examined for structural changes during luteolysis. Using fluorescence polarization, a rapid rigidification in vitro of samples obtained from ovaries undergoing spontaneous or prostaglandin F2 .alpha.-induced regression was observed. The rigidification, manifested by a 72% polarization increase over 50 min, is Ca and calmodulin dependent, temperature sensitive and protein mediated. This increase in polarization did not appear in fractions from nonregressing ovaries; however, addition of phospholipase A2 caused virtually identical changes in polarization results as in samples prepared from regressing ovaries. These results suggest that Ca-calmodulin-dependent phospholipase A2 plays a role in membrane deterioration during luteolysis.This publication has 17 references indexed in Scilit:
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