In Vitro Target Cell Lysis Mediated by Normal Human Lymphocytes

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Abstract
Lysis of DNP‐coated chicken erythrocytes by human blood lymphocytes (K cells) was induced by means of rabbit anti‐DNP antibodies. Antisera were prepared by injecting the animals with DNP‐conjugated proteins emulsified in Freund's complete adjuvant An ammonium sulphate precipitation technique was used for assay of antibody concentration and affinity. Sephadex G‐200 chromatography indicated that 90% of the DNP antibodies were 7S in the bleedings on days 10–16. whereas 99. 8% were 7S in later bleedings. 7S antibodies induced K‐cell lysis at high dilutions, whereas 19S antibodies were essentially negative Antibody fractions obtained by DF.AE‐ or CM‐cellulose chromatography were used to establish possible heterogeneities in the capacity of 7S antibodies to induce either K‐cell‐ or complement‐mediated target cell lysis. No such heterogeneities were found Fifteen IgG preparations containing antibodies of different affinities were compared with regard to their capacity to induce K‐cell‐mediated lysis. A statistically significant correlation was found between antibody affinity and efficiency in K‐cell‐mediated lysis. In a similar study of complement mediated lysis the correlation was not significant at the 5% level but was significant at the 10% level.

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