Purification of chicken intestinal receptor for 1 alpha, 25-dihydroxyvitamin D3 to apparent homogeneity.
- 1 January 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (1) , 16-20
- https://doi.org/10.1073/pnas.79.1.16
Abstract
The chicken intestinal 1.alpha.,25-dihydroxyvitamin D3 receptor-like protein has been purified to apparent homogeneity as determined by sodium dodecyl sulfate gel electrophoresis. The techniques employed for the purification include selective precipitation of the receptor by Polymin P (polyethyleneimine) and (NH4)2SO4 and batch adsorption to and selective elution from hydroxylapatite, followed by gel exclusion and DEAE-cellulose chromatography. The labeled receptor was eluted at a pH of .apprx. 6.0 on a chromatofocusing column. The protein was purified 6100-fold, and the receptor was obtained in 9% yield.This publication has 29 references indexed in Scilit:
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