Abstract
Postreplication repair and its inhibition by chloramphenicol and caffeine, as seen in alkaline sucrose gradients, were compared between a UV nonmutable strain uvrA umuC and normally mutable strains uvrA recF and uvrA umu + rec +of Escherichia coli K-12. The uvrA umuC strain performed postreplication repair as efficiently as the parental strain, while the repair in uvrA recF strain was dependent on UV dose. Both chloramphenicol and caffeine inhibited postreplication repair to an equal extent of about 25%, and 10%, respectively, in all three uvrA strains of umuC36, recF and umu + rec +. These observations suggest that postreplication repair is largely not responsible for UV mutagenesis.

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