A SIMPLE AND RAPID METHOD USING POLYETHYLENIMINE-TREATED FILTERS FOR ASSAY OF SOLUBILIZED LH/hCG RECEPTORS

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Abstract
Filtration through polyethylenimine (PEI)-treated membrane filters efficiently separates free radiolabeled human (h) LH [luteinizing hormone] or hCG [chorionic gonadotropin] from solubilized receptor-hormone complexes. Solubilized LH/hCG receptors of bovine, porcine and rat origin are suitable for measurement by this method. Specific binding assayed by the PEI-filter technique was 106-138% of specific binding assayed by double polyethylene glycol precipitation, demonstrating that the method gave quantitative recovery of bound hormone. Nonspecific binding was consistently lower with PEI-treated filters. Intra- and interassay variability for the 2 different methods was similar. Apparently, filtration through PEI-treated membrane filters is a simple and rapid method for assay of solubilized LH/hCG receptors.