Recognition by xenogeneic cytotoxic T lymphocytes of cells expressing HLA-A2 or HLA-B7 after DNA-mediated gene transfer.
- 1 August 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (16) , 5056-5060
- https://doi.org/10.1073/pnas.80.16.5056
Abstract
Murine L cells expressing HLA-A2 or -B7 antigens were isolated after cotransformation of thymidine kinase-negative cells with the herpes simplex virus thymidine kinase gene and the genomic clones containing either the HLA-A2 or -B7 genes. Monoclonal antibody binding analyses demonstrated the stable cell surface expression of HLA antigens by these cells at levels of up to 40% of the amount expressed by the human B lymphoblastoid cell line, JY. The HLA-A2 and -B7 antigens expressed by the L cells retained all of the antibody-defined, heavy-chain-associated antigenic determinants but lacked those determinants associated with human beta 2-microglobulin. These HLA transformants were capable of functioning as targets for monoclonal cytotoxic T lymphocytes (CTL) that specifically recognize the HLA-B7 or -A2 antigens expressed by JY cells. However, the efficiency of lysis, relative to the JY cell line, was 50-99% for individual CTL. In addition, not all of these CTL were capable of lysing the appropriate transformants. Because the antigens appear by serological criteria to be structurally intact and expressed at high levels, these results suggest that the complementation of the HLA heavy chains with mouse, rather than human, beta 2-microglobulin may alter the antigenic determinants that are important for CTL recognition.This publication has 29 references indexed in Scilit:
- Comparative structural analysis of HLA-A2 antigens distinguishable by cytotoxic T lymphocytes: variants M7 and DR1Biochemistry, 1982
- Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transferNature, 1982
- Monoclonal antibody (B27M2) subdividing HLA-B27Human Immunology, 1982
- Expression of marine H–2Kb histocompatibility antigen in cells transformed with cloned H–2 genesNature, 1982
- Use of HLA loss mutants to analyse the structure of the human major histocompatibility complexNature, 1982
- Clusters of genes encoding mouse transplantation antigensCell, 1982
- A pseudogene homologous to mouse transplantation antigens: Transplantation antigens are encoded by eight exons that correlate with protein domainsCell, 1981
- Major histocompatibility antigens: The human (HLA-A,-B,-C) and murine (H-2K, H-2D) class I moleculesCell, 1981
- BIOCHEMISTRY OF THE GENE PRODUCTS FROM MURINE MHC MUTANTSAnnual Review of Genetics, 1980
- Monoclonal Antibodies for Analysis of the HLA SystemImmunological Reviews, 1979