IDENTIFICATION AND CHARACTERIZATION OF 114-A10, AN ANTIGEN HIGHLY EXPRESSED ON THE SURFACE OF MURINE MYELOID AND ERYTHROID PROGENITOR CELLS AND IL-3-DEPENDENT CELL-LINES
- 1 September 1989
- journal article
- research article
- Vol. 17 (8) , 877-882
Abstract
Monoclonal antibody (mAb) 114/A10, raised against the murine bone marrow-derived multipotential hemopoietic progenitor cell line B6SUtA, identifies an antigen highly expressed by various interleukin-3 (IL-3)-dependent cell lines, the myelomonocytic cell line WEHI-3, and a large proportion of primary myeloid and erythroid colony-forming cells. Spleen- and bone marrow-derived 114/A10-positive cells were shown to selectively proliferate in vitro in response to pokeweed mitogen-stimulated spleen cell-conditioned medium or recombinant IL-3. Western blot analysis indicated that the antigen recognized by mAb 114/A10 has a mean relative molecular mass of approximately 150,000, although it is extremely heterogeneous is nature, and differs greatly in size range among different cell lines.This publication has 17 references indexed in Scilit:
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