Specific Binding Radioassay of Serum Thyroxine

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Abstract
Adaptation of the Murphy and Jachan method for serum thyroxine for routine use in a clinical chemistry laboratory is described. Details in the original paper have been amplified, and operations such as mixing, resin addition, and temperature control modified in the direction of greater precision and simplicity. A comparison study was made of the performance of the method in our laboratory with the reported performance in the laboratory of the original authors. In agreement with Murphy and coworkers and Cassidy et al, it was found that conditions could be established whereby the method was both reproducible and capable of yielding significant diagnostic information in the presence of iodinated radiographic compounds.

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