Luteinizing Hormone-Releasing Hormone Binds to Enriched Human Placental Membranes and Stimulates in Vitro the Synthesis of Bioactive Human Chorionic Gonadotropin*

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Abstract
Partially purified cell membranes were prepared from midterm and term placentas after sedimentation on a sucrose density gradient. Biochemical characterization showed that the sucrose density pellet was enriched 8-fold in alkaline phosphatase activity and also contained the majority of [125I] LHRH binding sites. This enrichment was also confirmed by electron microscopy. Specific binding of LHRH was then determined by incubating iodinated LHRH or two of its superanalogs with increasing doses of the corresponding radioinert ligand. Scatchard representation of the data showed curvilinear plots whose first component revealed, for both stages of pregnancy, saturable binding of [126I]LHRH and its agonists with similar association constants (Ka) that ranged between 5.5 × 106 M−1 and 1.1 × 107 M−1. When standardized per milligram of DNA content, the number of binding sites ranged between 225 and 310 × 10−12 M. Specificity was evidenced by the inability of a biologically active LHRH antagonist, oxytocin, and TRH to inhibit [126I]LHRH binding. Short term placental cultures incubated with 1.5 × 10−6 M LHRH had increased production rates of both immunoassayable and bioassayable hCG, and this effect was 4-fold higher in midterm placental cultures. Placental incubations with either buffer or equimolar concentrations of oxytocin or TRH had no effect on hCG production. These observations expand information on extrapituitary binding sites of LHRH and suggest a role for this peptide in the physiology of the human placenta.