H2B nucleohistone ? phospholipid interactions. Thermal denaturation and ultrastructural analysis
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 20 (3) , 159-166
- https://doi.org/10.1007/bf00243762
Abstract
Sphingomyelin, phosphatidylserine, bovine lecithin and phosphatidylethanolamine modify the thermal stability of H2B-DNA complexes, by inducing stabilization at 0.3 and 0.6 H2B:DNA weight ratios and destabilization at 0.8, 1.0 and 1.5 ones. The ability of phospholipids to modify the arrangement of nucleohistone is confirmed by ultrastructural analysis which indicates a competitive action of these molecules during the nucleoprotein assembly. A possible regulatory role of phospholipids on native chromatin is proposed.Keywords
This publication has 43 references indexed in Scilit:
- Phospholipids bound to acidic nuclear proteins in human B and T lymphocytesMolecular and Cellular Biochemistry, 1976
- Lipid-F1 nucleohistone interactionsMolecular and Cellular Biochemistry, 1976
- New DNA polymerase activity associated with nuclear membranes from a human lymphoid cell lineBiochemical and Biophysical Research Communications, 1975
- Synthesis of glycolipids and phospholipids in hamster cells: Dependence on cell density and the cell cycleJournal of Cellular Physiology, 1975
- On the mechanism of interaction between histone II B1 and DNA and histone II B2 and DNAJournal of Theoretical Biology, 1974
- Identification of a nuclear protein matrixBiochemical and Biophysical Research Communications, 1974
- Ultrastructural and biochemical characteristics of reconstructed chromatin and synthetic nucleohistonesExperimental Cell Research, 1973
- A super-coil model for nucleohistoneJournal of Molecular Biology, 1972
- Nature of the DNA associated with the nuclear envelope of regenerating liverJournal of Molecular Biology, 1971
- Optical absorbance and optical rotatory dispersion studies on calf thymus nucleohistoneJournal of Molecular Biology, 1969