A Preliminary Analysis of the Proteins of the Primary Walls of some Plant Cells

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Abstract
Amino-acid analyses on hydrolysates of the wall, microsomal, and soluble fractions of Jerusalem artichoke tubers, pea stems, and oat coleoptiles showed that the wall fractions alone contained hydroxyproline. In the case of Jerusalem artichoke, this substance was positively identified as 1-hydroxyproline. In the electron microscope, contamination of the wall fraction by cytoplasm and cytoplasmic membrane was found to be negligible. Analyses on Jerusalem artichoke tuber tissue showed that the amounts of protein and hydroxyprolme in the walls increased with growth. It is concluded that a distinct group of proteins containing hydroxyproline form an integral part of the primary walls of these tissues.