Partial Purification of Proteinase Inhibitors from Tomato Plants Infected withPhytophthora infestans

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Abstract
Chymotrypsin and trypsin inhibitors were partially purified from the leaves of tomato plants (L. esculentum) by affinity chromatography and isoelectric focusing 48 h after inoculation with P. infestans, tomato race O. Tomato selections 33 (TS 33), incompatible, and 19 (TS 19), compatible, to tomato race O of P. infestans were used in the study. The initial inhibitor activity was greater in uninoculated plants of TS 33 than in TS 19, and overall inhibitor activity declined in both tomato selections after inoculation. Measurements of individual inhibitor activity, after isoelectric focusing, showed that there was a decline in the levels of most inhibitors following inoculation. The same inhibitors were detected in inoculated and uninoculated tomato leaves. The results from this study failed to corroborate a previous report from this laboratory than an increase in proteinase inhibitor activity in crude extracts was associated with the incompatible response of TS 33 inoculated with race O of P. infestans.