Determination of Free Haemoglobin in Serum by an Automated Assay Using 4-Aminophenazone and the Cobas Bio System
- 1 January 1981
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 19 (9) , 971-976
- https://doi.org/10.1515/cclm.1981.19.9.971
Abstract
A colorimetric method for the determination of free Hb in human serum is described, using 4-aminophenazone as chromogen, and commercially available hemiglobin cyanide solutions as standards. The reaction system is suitable for manual measurements as well as determinations with a centrifugal analyzer (Cobas Bio System). Accuracy, linearity and stability of the procedure is shown. A reference interval in sera of normal adults was established, with an upper limit of 5 mg/dl. The alternative use of heparin plasma instead of serum is discussed.This publication has 7 references indexed in Scilit:
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