Rapid Screening Method for Aflatoxins and Zearalenone in Corn
- 1 January 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 58 (1) , 114-116
- https://doi.org/10.1093/jaoac/58.1.114
Abstract
The methanol-water extraction system used in AOAC Method II for aflatoxins extracts both the aflatoxins and zearalenone from corn. Using this methanol-water extraction system as a base, a rapid screening procedure has been developed for these mycotoxins. The methanol-water extract is defatted with hexane and the pigments are precipitated with copper carbonate. The aflatoxins and zearalenone are subsequently extracted into chloroform and are then detected by half-plate TLC. An elapsed time of about 1 hr is required to analyze 1 sample. The sensitivity of the method is about 2 μg/kg for aflatoxin B1 and 100 μg/kg for zearalenone.Keywords
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