Storage of Red Cells in a CPD/SAGM System Using Teruflex® PVC

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Abstract
A quadruple plastic bag system made of Teruflex PVC [polyvinyl chloride] (Terumo) was tested. Red cells separated from plasma and buffy coat were resuspended in SAGM [saline adenine-glucose mannitol] solution. After storage for 42 days, the 24-h posttransfusion autologous survival was 73.3 .+-. 6.6% (.hivin.X .+-. SD), range 61.8-80.7%, n = 10. The shape of the cells was changed so that normal or slightly abnormal morphology was found in 37 .+-. 7% of the cells after 42 days. Spontaneous in vitro hemolysis was 0.63 .+-. 0.32% (range 0.28-1.13). A sufficient glucose reserve still remained at the end of storage, but glucose consumption and lactate production were impaired during the last 2 wk due to the increasing acidity. By extrapolation from other studies it is suggested that 35 days is a more suitable shelf life than 42 days in this system.